du.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • chicago-author-date
  • chicago-note-bibliography
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Simultaneous quantification of the highly lipophilic atovaquone and the hydrophilic strong basic proguanil and its metabolites, using a new mixed-mode SPE approach and Steep-Gradient LC
Högskolan Dalarna, Akademin Industri och samhälle, Kemiteknik.
Högskolan Dalarna, Akademin Industri och samhälle, Kemiteknik.
2005 (engelsk)Inngår i: Journal of Chromatographic Science, ISSN 0021-9665, E-ISSN 1945-239X, Vol. 43, nr 5, s. 259-266Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

A bioanalytical method is described for the simultaneous quantitative analysis of the highly lipophilic atovaquone and the strong basic proguanil with metabolites in plasma. The drugs are extracted from protein precipitated plasma samples on a novel mixed-mode solid-phase extraction (SPE) column containing carboxypropyl and octyl silica as functional groups. The analytes are further separated and quantitated using a steep-gradient liquid chromatograhic method on a Zorbax SB-CN column with UV detection at 245 nm. Two different internal standards (IS) are used in the method to compensate for both types of analytes. A structurally similar IS to atovaquone is added with acetonitrile to precipitate proteins from plasma. A structurally similar IS to proguanil and its metabolites is added with phosphate buffer before samples are loaded onto the SPE columns. A single elution step is sufficient to elute all analytes. The method is validated according to published guidelines and shows excellent performance. The within-day precisions, expressed as relative standard deviation, are lower than 5% for all analytes at three tested concentrations within the calibration range. The between-day precisions are lower than 13% for all analytes at the same tested concentrations. The limit of quantitation is 25nM for the basic substances and 50nM for atovaquone. Several considerations regarding development and optimization of a method for determination of analytes with such a difference in physiochemical properties are discussed.

sted, utgiver, år, opplag, sider
2005. Vol. 43, nr 5, s. 259-266
HSV kategori
Identifikatorer
URN: urn:nbn:se:du-1203DOI: 10.1093/chromsci/43.5.259ISI: 000229224600007PubMedID: 15975245OAI: oai:dalea.du.se:1203DiVA, id: diva2:519533
Tilgjengelig fra: 2005-06-01 Laget: 2005-06-01 Sist oppdatert: 2017-12-07bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMed

Søk i DiVA

Av forfatter/redaktør
Blessborn, DanielBergqvist, Yngve
Av organisasjonen
I samme tidsskrift
Journal of Chromatographic Science

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 547 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • chicago-author-date
  • chicago-note-bibliography
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf