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Determination of melatonin in saliva using solid-phase extraction, high-performance liquid chromatography and fluorescence detection
Dalarna University, School of Technology and Business Studies, Chemical Engineering.
Dalarna University, School of Education, Health and Social Studies, Medical Science.
2006 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 66, no 3, p. 181-190Article in journal (Refereed) Published
Abstract [en]

A sensitive bioanalytical method for the determination of melatonin in saliva by solid-phase extraction (SPE), high-performance liquid chromatography (HPLC) and fluorescence detection has been developed and validated. Saliva was collected with a Salivette((R)) sampling device (Sarstedt) and a mixed-mode SPE column was used for the extraction of melatonin and internal standard (N-acetyl-6-methoxytryptamine) from the saliva. Chromatographic separation was performed using a HyPurity C18 LC column (150x2.1 mm) with mobile phase acetonitrile-ammonium hydrogen carbonate buffer, 0.015 M, pH 6.8 (23:77, v/v). Excitation and emission wavelengths were set to 285 nm and 345 nm, respectively. The within-day precision for the method at 50 pmol/L was 7.9% and the between-day precision was 10.5%. The limit of quantification was 50 pmol/L.

Place, publisher, year, edition, pages
2006. Vol. 66, no 3, p. 181-190
Keywords [en]
circadian rhythm; HPLC; indoleamine; N-acetyl-5-methoxytryptamine; salivette; SPE
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:du-596DOI: 10.1080/00365510600548777ISI: 000237606700002PubMedID: 16714247OAI: oai:dalea.du.se:596DiVA, id: diva2:519393
Available from: 2004-12-02 Created: 2004-12-02 Last updated: 2017-12-07Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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  • vancouver
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  • Other style
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  • nn-NB
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