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Development and validation of a bioanalytical method using automated solid-phase extraction and LC-UV for the simultaneous determination of lumefantrine and its desbutyl metabolite in plasma
Dalarna University, School of Technology and Business Studies, Chemical Engineering.
Dalarna University, School of Technology and Business Studies, Chemical Engineering.
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2005 (English)In: Journal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, E-ISSN 1873-264X, Vol. 37, no 5, p. 1081-1088Article in journal (Refereed) Published
Abstract [en]

A bioanalytical method for the determination of lumefantrine (LF) and its metabolite desbutyl-lumefantrine (DLF) in plasma by solid-phase extraction (SPE) and liquid chromatography has been developed. Plasma proteins were precipitated with acetonitrile: acetic acid (99:1, v/v) containing a DLF analogue internal standard before being loaded onto a octylsilica (3 M Empore) SPE column. Two different DLF analogues were evaluated as internal standards. The compounds were analysed by liquid chromatography UV detection on a SB-CN (250 mm x 4.6 mm) column with a mobile phase containing acetonitrile-sodium phosphate buffer pH (2.0; 0.1 M) (55:45, v/v) and sodium perchlorate 0.05 M. Different SPE columns were evaluated during method development to optimise reproducibility and recovery for LF, DLF and the two different DLF analogues. The within-day precisions for LF were 6.6 and 2.1% at 0.042 and 8.02 μ g/mL, respectively, and for DLF 4.5 and 1.5% at 0.039 and 0.777 μ g/mL, respectively. The between-day precisions for LF were 12.0 and 2.9% at 0.042 and 8.02 μ g/mL, respectively, while for DLF 0.7 and 1.2% at 0.039 and 0.777 μ g/mL, respectively. The limit of quantification was 0.024 and 0.021 μ g/mL for LF and DLF, respectively. Different amounts of lipids in plasma did not affect the absolute recovery of LF or DLF.

Place, publisher, year, edition, pages
2005. Vol. 37, no 5, p. 1081-1088
Keywords [en]
antimalarial; lumefantrine; lipophilic; liquid chromatography; solid-phase extraction; stability; validation
National Category
Chemical Engineering
Identifiers
URN: urn:nbn:se:du-1202DOI: 10.1016/j.jpba.2004.07.041ISI: 000229599200034PubMedID: 15862688OAI: oai:dalea.du.se:1202DiVA, id: diva2:519532
Available from: 2005-06-01 Created: 2005-06-01 Last updated: 2017-12-07Bibliographically approved

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