Background: The best birthing position for women to adopt during labour and birth is to vary the positions freely depending on the stages of labour. The aim of this study was to define the concept ‘upright position during labour and birth’ through the synthesis of texts from literature and interview data with Bangladeshi health care providers, in an attempt to clarify its meaning, enabling comprehension and use of the concept in clinical practice and education.Methods: This study design was concept analysis. The setting was three different nursing institutes/colleges and nearby hospitals. Nine participants were invited to participate in an individual in-depth interview (IDI) with semi-structured open-ended questions. A concept analysis was undertaken according to the hybrid model which consists of the theoretical, fieldwork and analytical phases. After a literature review, the concept was empirically clarified in the fieldwork phase in the midwifery context of Bangladesh, described with the Quality Maternal and Newborn Care (QMNC) framework components.Results: The concept of upright position during labour and birth in the midwifery context was redefined. Altering between positions widening the pelvic relief labour pain for the woman, potentially resulting in a normal birth with a healthy mother and baby. Women do not expect upright positions in facility-based births but at homebirths.Conclusion: The women’s rights to spontaneously and freely move while in labour, altering between different vertical positions are associated with the midwives’ work “with women” supporting the women.Clinical implications: It is a simple intervention to apply in clinical setting and simple to teach to nurses, midwives and physicians and in graduate and postgraduate education.