The sperm in the seminal fluid is affected by various environmental factors, where one is the pH-value in the environment. An extremely low or high pH-value has been observed in other studies to be lethal to sperm cells, thus effecting the sperms capability to perform a conception. Addition of nutrion, such as glucose and fructose, also has been a factor contributing to the survival of the sperm. In this essay we found that the pH-value did not affect the lifespan of the sperm. Mainly due to the pH-value in the seminal fluid and the buffer capacity of the same, the different pH-solutions we used showed no significant effect on the sperm’s lifespan. Addition of nutrion (glucose or fructose) did however affect the time the sperm survived, adding hours to the lifespan. The best media for survival was the IVFTM nutrion solution used to perform so called ”swim up” tests, where the sperms get ”washed” and used without the seminal fluid’s content. The IVFTM improved the motility of the sperms and also made them longer living.