This thesis investigates how the optical properties of paperboard influences printing with targets in ISO 12647-2. The targets for optical properties in ISO 12647-2 are defined for paperboard without optical brightening agents and fluorescent brightening agents wich makes it difficult to reach the targets when printing paperboard containing these agents. Seven different types of paperboard, some with and some without the agents, have been printed and instrumentally and visually measured to see if there is any deviation from the standard targets and if it shows visually. The result shows that paperboard containing optical brightening agents and fluorescent brightening agents can print with targets in ISO 12647-2 but in many measurments the difference between the types of paperboard were to small to assess. The differences between the types of paperboard in the instrumental measurements did not fully correspond with the way the panel appraised them visually.