du.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • chicago-author-date
  • chicago-note-bibliography
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Importance of pre-analytical factors contributing to measurement uncertainty, when determining sulfadoxine and sulfamethoxazole from capillary blood dried on sampling paper.
Dalarna University, School of Technology and Business Studies, Chemical Engineering.
Dalarna University, School of Technology and Business Studies, Chemical Engineering.
2008 (English)In: Journal of Chromatographic Science, ISSN 0021-9665, E-ISSN 1945-239X, Vol. 46, no 9, p. 837-843Article in journal (Refereed) Published
Abstract [en]

A bioanalytical method is developed and validated for determination of sulfadoxine (SD) and sulfamethoxazole (SM) in 100 µL capillary blood dried on sampling paper (Whatman 31ET Chr). SD and SM are extracted with 2000 µL perchloric acid and the liquid phase is loaded onto ENV+ solid-phase extraction columns. SD, SM, and the internal standard are separated on a Purospher STAR RP-18 liquid chromatography column (150 × 4.6 mm) with a mobile phase consisting of acetonitrile–sodium acetate buffer pH 5.2, I = 0.1 (33:67, v/v). Analytes are detected with UV at 256 nm. Lower limit of quantitation is 5 µmol/L, where precisions are 4.2% and 3.9% for SD and SM, respectively. Three brands of sampling papers have been compared with respect to absorption properties, extraction recoveries, and variations. Punching out dried blood spots (DBS) instead of cutting spots into strips prior to extraction has been evaluated by examining precision and accuracy of SD and SM determinations. Importance of uniformity of types of sampling paper, sampling volume and biological matrix, benefit of punching out discs from DBS, and impact on absorption properties of different brands of sampling papers are discussed. Avoiding pre-analytical errors whenever possible results in concentrations determined being more accurate and precise.

Place, publisher, year, edition, pages
2008. Vol. 46, no 9, p. 837-843
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:du-2668ISI: 000260912400001PubMedID: 19007488OAI: oai:dalea.du.se:2668DiVA, id: diva2:519797
Available from: 2007-04-04 Created: 2007-04-04 Last updated: 2017-12-07Bibliographically approved

Open Access in DiVA

No full text in DiVA

PubMed

Search in DiVA

By author/editor
Malm, MikaelaBergqvist, Yngve
By organisation
Chemical Engineering
In the same journal
Journal of Chromatographic Science
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric score

pubmed
urn-nbn
Total: 543 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • chicago-author-date
  • chicago-note-bibliography
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf