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Development and validation of an automated solid-phase extraction and liquid chromatographic method for determination of lumefantrine in capillary blood on sampling paper.
Dalarna University, School of Technology and Business Studies, Chemical Engineering.
Dalarna University, School of Technology and Business Studies, Chemical Engineering.
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2007 (English)In: Journal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, E-ISSN 1873-264X, Vol. 45, no 2, p. 282-287Article in journal (Refereed) Published
Abstract [en]

A bioanalytical method for the determination of lumefantrine in 100 µl blood applied onto sampling paper, by solid-phase extraction and liquid chromatography, has been developed and validated. Whatman 31 ET Chr sampling paper was pre-treated with 0.75 M tartaric acid before sampling capillary blood to enable a high recovery of lumefantrine. Lumefantrine was extracted from the sampling paper, then further purified using solid-phase extraction and finally quantified with HPLC. The between-day variation was below 10% over the range 0.4–25 µM. The lower limit of quantification was 0.25 µM in 100 µl capillary blood. No decrease in lumefantrine concentration in dried blood spot is seen after 4 months storage at 22 °C. The method was also evaluated in field samples from patients in Tanzania after treatment with lumefantrine/artemether. Lumefantrine could be estimated accurately enough to assess bioavailability and treatment compliance on day 7 (i.e. 4 days after the last dose) after a standard regimen with the lumefantrine/artemether combination.

Place, publisher, year, edition, pages
2007. Vol. 45, no 2, p. 282-287
Keywords [en]
lumefantrine; sampling paper; dried blood spots; capillary blood; antimalarial drugs; solid-phase extraction; liquid chromatography
National Category
Chemical Engineering
Identifiers
URN: urn:nbn:se:du-2956DOI: 10.1016/j.jpba.2007.07.015ISI: 000250888700014PubMedID: 17719735OAI: oai:dalea.du.se:2956DiVA, id: diva2:519877
Available from: 2007-10-14 Created: 2007-10-14 Last updated: 2017-12-07Bibliographically approved

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Blessborn, DanielRömsing, SusanneBergqvist, Yngve
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CiteExportLink to record
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Citation style
  • apa
  • ieee
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  • vancouver
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  • de-DE
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