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Determination of eflornithine enantiomers in plasma by precolumn derivatization with o-phthalaldehyde-N-acetyl-L-cysteine and liquid chromatography with UV detection
Dalarna University, School of Education, Health and Social Studies, Chemistry.
2010 (English)In: BMC Biomedical chromotography, ISSN 0269-3879, E-ISSN 1099-0801, Vol. 24, no 7, 768-773 p.Article in journal (Refereed) Published
Abstract [en]

A bioanalytical method for indirect determination of eflornithine enantiomers in 75?µL human plasma has been developed and validated. l- and d-eflornithine were derivatized with o-phthalaldehyde and N-acetyl-L-cysteine to generate diastereomers which were separated on two serially connected Chromolith Performance columns (RP-18e 100 × 4.6?mm i.d.) by a isocratic flow followed by a gradient flow for elution of endogenous compounds. The diastereomers were detected with UV (340?nm). The between-day precisions for L- and D-eflornithine in plasma were 8.4 and 2.3% at 3?µm, 4.0 and 5.1% at 400?µm, and 2.0 and 3.7% at 1000?µm. The lower limit of quantification was determined to be 1.5?µm, at which precision was 14.9 and 9.9% for L- and D-eflornithine, respectively

Place, publisher, year, edition, pages
Wiley , 2010. Vol. 24, no 7, 768-773 p.
Keyword [en]
eflornithine; chiral separation; Human African sleeping sickness; cancer
National Category
Chemical Engineering
Identifiers
URN: urn:nbn:se:du-5207DOI: 10.1002/bmc.1361ISI: 000279367900013OAI: oai:dalea.du.se:5207DiVA: diva2:520299
Available from: 2011-01-17 Created: 2011-01-17 Last updated: 2015-06-12Bibliographically approved

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