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Assay for screening for six antimalarial drugs and one metabolite using dried blood spot sampling, sequential extraction and ion-trap detection
Dalarna University, School of Education and Humanities, Chemistry.
Dalarna University, School of Education and Humanities, Chemistry.
2010 (Undetermined)In: Bioanalysis, ISSN 1757-6199, Vol. 2, no 10, 1839-1847 p.Article in journal (Refereed) Published
Abstract [un]

Background: More parasites are becoming resistant to antimalarial drugs, and in many areas a change in first-line drug treatment is necessary. The aim of the developed assay is to help determine drug use in these areas and also to be a complement to interviewing patients, which will increase reliability of surveys. Results: This assay detects quinine, mefloquine, sulfadoxine, pyrimethamine, lumefantrine, chloroquine and its metabolite desethylchloroquine in a 100-µl dried blood spot. Most of the drugs also have long half-lives that make them detectable at least 7 days after administration. The drugs are extracted from the dried blood spot with sequential extraction (due to the big differences in physicochemical properties), solid-phase extraction is used as sample clean-up and separation is performed with gradient-LC with MS ion-trap detection. Conclusion: Detection limits (S/N > 5:1) at 50 ng/ml or better were achieved for all drugs except lumefantrine (200 ng/ml), and thus can be used to determine patient compliance. A major advantage of using the ion-trap MS it that it will be possible to go back into the data and look for other drugs as needed

Place, publisher, year, edition, pages
Future Science Ltd , 2010. Vol. 2, no 10, 1839-1847 p.
Identifiers
URN: urn:nbn:se:du-5209OAI: oai:dalea.du.se:5209DiVA: diva2:520300
Available from: 2011-01-17 Created: 2011-01-17 Last updated: 2012-04-24Bibliographically approved

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