The aim of this paper is to introduce the innovative technology created within the framework of PRE-FOREST project, funded by the European Commission under the CRAFT 6th Framework Research Programme. PRE-FOREST was an innovative research project in the field of forest planting stock material, enhancing co-operation between research foundations and small and medium-sized enterprises. Among the project's objectives were the introduction of a new technology based on precultivation (in mini-plugs) of forest regeneration material in a cost-efficient and environmental friendly production unit, not affected by outdoor climate; the development of a new, adapted to mini-plugs, grading technology and replug robot; the integration of these technologies into a functional prototype unit capable of large-scale forest regeneration material production; and the introduction of this production system at strategic locations in Europe. The system has been developed for year-round production and function in the same way, independently of climatic variations in different parts of Europe. To obtain these objectives the study focused on the interaction among production technology, mini-plug container design and rooting media, to develop cultivation protocols for forest reproductive materials of special economic and ecological importance for each participating country. One of the main benefits of the new technology was the environmentally friendly production of a large number of seedlings per square metre, leading to a cost-efficient result.