du.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • chicago-author-date
  • chicago-note-bibliography
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Assay for screening for six antimalarial drugs and one metabolite using dried blood spot sampling, sequential extraction and ion-trap detection
Dalarna University, School of Technology and Business Studies, Chemical Engineering.
Dalarna University, School of Education, Health and Social Studies, Chemistry.
Dalarna University, School of Education, Health and Social Studies, Medical Science.
2010 (English)In: Bioanalysis, ISSN 1757-6180, E-ISSN 1757-6199, Vol. 2, no 11, 1839-1847 p.Article in journal (Refereed) Published
Abstract [en]

Background: More parasites are becoming resistant to antimalarial drugs, and in many areas a change in first-line drug treatment is necessary. The aim of the developed assay is to help determine drug use in these areas and also to be a complement to interviewing patients, which will increase reliability of surveys.

Results: This assay detects quinine, mefloquine, sulfadoxine, pyrimethamine, lumefantrine, chloroquine and its metabolite desethylchloroquine in a 100-mu l dried blood spot. Most of the drugs also have long half-lives that make them detectable at least 7 days after administration. The drugs are extracted from the dried blood spot with sequential extraction (due to the big differences in physicochemical properties), solid-phase extraction is used as sample clean-up and separation is performed with gradient-LC with MS ion-trap detection.

Conclusion: Detection limits (S/N > 5:1) at 50 ng/ml or better were achieved for all drugs except lumefantrine (200 ng/ml), and thus can be used to determine patient compliance. A major advantage of using the ion-trap MS it that it will be possible to go back into the data and look for other drugs as needed.

Place, publisher, year, edition, pages
2010. Vol. 2, no 11, 1839-1847 p.
National Category
Clinical Medicine Chemical Engineering
Research subject
Hälsa och välfärd
Identifiers
URN: urn:nbn:se:du-10465DOI: 10.4155/BIO.10.147ISI: 000286647100016OAI: oai:DiVA.org:du-10465DiVA: diva2:542756
Available from: 2012-08-03 Created: 2012-08-03 Last updated: 2015-09-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Blessborn, DanielRömsing, SusanneBergqvist, Yngve
By organisation
Chemical EngineeringChemistryMedical Science
In the same journal
Bioanalysis
Clinical MedicineChemical Engineering

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 651 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • chicago-author-date
  • chicago-note-bibliography
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf