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A novel combination of TaqMan RT-PCR and a suspension microarray assay for the detection and species identification of pestiviruses
Dalarna University, School of Education, Health and Social Studies, Medical Science.
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2010 (English)In: Veterinary Microbiology, ISSN 0378-1135, E-ISSN 1873-2542, Vol. 142, no 1-2, 81-86 p.Article in journal (Refereed) Published
Abstract [en]

The genus pestivirus contains four recognized species: classical swine fever virus, border disease virus, bovine viral diarrhoea virus types 1 and 2. All are economically important and globally distributed but classical swine fever is the most serious, concerning losses and control measures. It affects both domestic pigs and wild boars. Outbreaks of this disease in domestic pigs call for the most serious measures of disease control, including a stamping out policy in Europe. Since all the members of the pestivirus genus can infect swine, differential diagnosis using traditional methods poses some problems. Antibody tests may lack specificity due to cross-reactions, antigen capture ELISAs may have low sensitivity, and virus isolation may take several days or even longer time to complete. PCR-based tests overcome these problems for the most part, but in general lack the multiplexing capability to detect and differentiate all the pestiviruses simultaneously. The assay platform described here addresses all of these issues by combining the advantages of real-time PCR with the multiplexing capability of microarray technology. The platform includes a TaqMan real-time PCR designed for the universal detection of pestiviruses and a microarray assay that can use the amplicons produced in the real-time PCR to identify the specific pestivirus. (C) 2009 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
2010. Vol. 142, no 1-2, 81-86 p.
National Category
Clinical Medicine
Identifiers
URN: urn:nbn:se:du-10490DOI: 10.1016/j.vetmic.2009.09.046ISI: 000276800800012OAI: oai:DiVA.org:du-10490DiVA: diva2:542782
Conference
7th Pestivirus Symposium of the European-Society-of-Veterinary-Virology, SEP 17-19, 2008, Uppsala, SWEDEN
Available from: 2012-08-03 Created: 2012-08-03 Last updated: 2015-06-12Bibliographically approved

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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • chicago-author-date
  • chicago-note-bibliography
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf