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Ammonium chloride ingestion increases resting mRNA content but attenuates exercise-induced mRNA levels in human skeletal muscle
Massey University, New Zealand.
Centre of Inflammation and Metabolism (CIM), Department of Molecular Biology, University of Copenhagen, Denmark.
Dalarna University, School of Education, Health and Social Studies, Sport and Health Science.
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2015 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, no 12, e0141317Article in journal (Refereed) Published
Abstract [en]

Minimizing the decrease in intracellular pH during high-intensity exercise training promotes greater improvements in mitochondrial respiration. This raises the intriguing hypothesis that pH may affect the exercise-induced transcription of genes that regulate mitochondrial biogenesis. Eight males performed 10x2-min cycle intervals at 80%  intensity on two occasions separated by ~2 weeks. Participants ingested either ammonium chloride (ACID) or calcium carbonate (PLA) the day before and on the day of the exercise trial in a randomized, counterbalanced order, using a crossover design. Biopsies were taken from the vastus lateralis muscle before and after exercise. The mRNA level of peroxisome proliferator-activated receptor co-activator 1α (PGC-1α), citrate synthase, cytochome c and FOXO1 was elevated at rest following ACID (P<0.05). During the PLA condition, the mRNA content of mitochondrial- and glucose-regulating proteins was elevated immediately following exercise (P<0.05). In the early phase (0–2 h) of post-exercise recovery during ACID, PGC-1α, citrate synthase, cytochome C, FOXO1, GLUT4, and HKII mRNA levels were not different from resting levels (P>0.05); the difference in PGC-1α mRNA content 2 h post-exercise between ACID and PLA was not significant (P = 0.08). Thus, metabolic acidosis abolished the early post-exercise increase of PGC-1α mRNA and the mRNA of downstream mitochondrial and glucose-regulating proteins. These findings indicate that metabolic acidosis may affect mitochondrial biogenesis, with divergent responses in resting and post-exercise skeletal muscle.

Place, publisher, year, edition, pages
2015. Vol. 10, no 12, e0141317
National Category
Clinical Medicine
Research subject
Health and Welfare
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URN: urn:nbn:se:du-14436DOI: 10.1371/journal.pone.0141317OAI: oai:DiVA.org:du-14436DiVA: diva2:727225
Available from: 2014-06-19 Created: 2014-06-19 Last updated: 2017-06-05Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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