This thesis investigates the relationship between travel motivation to natural areas measured by a Recreation Experience Preference (REP) scale and environmental awareness measured by the New Environmental Paradigm (NEP) scale. The primary data for the research were gained from a quantitative online survey focusing on students of the University Palacký in Olomouc, Czech Republic. The thesis handles the definition of nature tourism, presents basic approaches to investigation of travel motivation, research on travel motivation in the Czech Republic, a brief overview of the nature potential for tourism in the Czech Republic and also the relationship between the environment and tourism. Factor analysis was the key method for data analysis. Through this method factors for individual scales were created and these factors were further used as new variables. It was found that internal motivation exceeds external motives and that higher environmental awareness of respondents is related to higher motivation to nature tourism.