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  • 51.
    Radoglou, Kalliopi
    et al.
    Democritus University of Thrace.
    Sismanis, Nikos
    Democritus University of Thrace.
    Smirnakou, Sonia
    Democritus University of Thrace.
    Mattsson, Anders
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Hernandez Velasco, Marco
    Dalarna University, School of Technology and Business Studies, Energy Technology.
    Schirone, Bartolomeo
    Universita degli Studi della Tuscia.
    Marras, Tatiana
    Universita degli Studi della Tuscia.
    Vessella, Federico
    Universita degli Studi della Tuscia.
    Chiatante, Donato
    Università degli Studi dell'Insubria.
    Terzaghi, Mattia
    Università degli Studi dell'Insubria.
    Fulgaro, Nicoletta
    Università degli Studi dell'Insubria.
    Montagnoli, Antonio
    Università degli Studi dell'Insubria.
    ZEPHYR Project – Deliverable D3.2: Intermediate report on growth tests2014Report (Other (popular science, discussion, etc.))
  • 52.
    Stattin, Eva
    et al.
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Lindström, Anders
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Användarvänligt och snabbt vitalitetstest2008Report (Other academic)
  • 53.
    Stattin, Eva
    et al.
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Lindström, Anders
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Elektrolytiskt läckage visar plantornas vitalitet2008In: Plantaktuellt, ISSN 1403-3321, no 4Article in journal (Refereed)
  • 54.
    Stattin, Eva
    et al.
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Lindström, Anders
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Rejält hett i kartongen!2008In: Plantaktuellt, ISSN 1403-3321, no 3Article in journal (Refereed)
  • 55.
    Stattin, Eva
    et al.
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Lindström, Anders
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Resultat från torktest av plantor - Delrapport 5: Långtidsuppföljning av plantor efter värmebehandling2007Report (Other academic)
  • 56.
    Stattin, Eva
    et al.
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Lindström, Anders
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Nathalie, Verhoef
    Balk, Peter
    Quality management for vital forest tree seedlings: Final report, Project no: P33854-12011Report (Other academic)
    Abstract [en]

    The work concerns development of a prototype molecular tests to identify vitality status of conifer seedlings. The work is done by NSure, Holland, Dalarna University and SUAS. In case for spruce, a successful validation experiment has been performed to validate the identified frost tolerance and vitality genes. Multiple indicators were identified that can be used to either reinforce the existing ColdnSure test, but also for development of a vitality test. The identified frost tolerance and vitality genes for pine still need to be validated. NSure together with Dalarna University aim to perform a validation next season. Multiple LN indicators were identified in spruce that can be used to determine the effectiveness of a LN treatment, but they are not yet validated. In spruce and pine hardly any scientific research is performed to study the effect of a LN treatment, particularly not at molecular level. Therefore NSure together with Dalarna Research Station want to apply for a project. Within this project, we would be able to develop the tests further.

  • 57.
    Stattin, Eva
    et al.
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Lindström, Anders
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Vemhäll, Marianne
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    RGC-analys av 5 tallpartier från Lugnets plantskola2009Report (Other academic)
  • 58.
    Stattin, Eva
    et al.
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Lindström, Anders
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Vemhäll, Marianne
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    RGC-analys av 5 tallpartier från Lugnets plantskola2010Report (Other academic)
  • 59.
    Stattin, Eva
    et al.
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Lindström, Anders
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Vemhäll, Marianne
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    RGC-analys, Lugnets plantskola2011Report (Other academic)
  • 60.
    Stattin, Eva
    et al.
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Verhoef, Nathalie
    Balk, Peter
    van Wordragen, Monique
    Lindström, Anders
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Development of a molecular test to determine the vitality status of Norway spruce (Picea abies) seedlings during frozen storage2012In: New forests, ISSN 0169-4286, E-ISSN 1573-5095, Vol. 43, no 5-6, p. 665-678Article in journal (Refereed)
    Abstract [en]

    In boreal forest regions, a great portion of forest tree seedlings are stored indoors in late autumn to prevent seedlings from outdoor winter damage. For seedlings to be able to survive in storage it is crucial that they store well and can cope with the dark and cold storage environment. The aim of this study was to search for genes that can determine the vitality status of Norway spruce (Picea abies (L.) Karst.) seedlings during frozen storage. Furthermore, the sensitivity of the ColdNSure (TM) test, a gene activity test that predicts storability was assessed. The storability of seedlings was tested biweekly by evaluating damage with the gene activity test and the electrolyte leakage test after freezing seedlings to -25 A degrees C (the SELdiff-25 method). In parallel, seedlings were frozen stored at -3 A degrees C. According to both methods, seedlings were considered storable from week 41. This also corresponded to the post storage results determined at the end of the storage period. In order to identify vitality indicators, Next Generation Sequencing (NGS) was performed on bud samples collected during storage. Comparing physiological post storage data to gene analysis data revealed numerous vitality related genes. To validate the results, a second trial was performed. In this trial, gene activity was better in predicting seedling storability than the conventional freezing test; this indicates a high sensitivity level of this molecular assay. For multiple indicators a clear switch between damaged and vital seedlings was observed. A collection of indicators will be used in the future development of a commercial vitality test.

  • 61.
    Wallin, Elisabeth
    et al.
    Dalarna University, School of Technology and Business Studies, Energy Technology. SLU.
    Gräns, Daniel
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology. SLU.
    Jacobs, Douglass F
    Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA..
    Lindström, Anders
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology. SLU.
    Verhoef, Nathalie
    NSure, Binnenhaven 5, NL-6700 AA Wageningen, Netherlands..
    Short-day photoperiods affect expression of genes related to dormancy and freezing tolerance in Norway spruce seedlings2017In: Annals of Forest Science, ISSN 1286-4560, E-ISSN 1297-966X, Vol. 74, no 3, article id 59Article in journal (Refereed)
    Abstract [en]

    Key message: Gene expression analysis showed that prolonged short day (SD) treatment deepened dormancy and stimulated development of freezing tolerance of Picea abies seedlings. Prolonged SD treatment also caused later appearance of visible buds in autumn, reduced risks for reflushing, and promoted earlier spring bud break.

    Context: Short day (SD) treatment of seedlings is a common practice in boreal forest tree nurseries to regulate shoot growth and prepare the seedlings for autumn planting or frozen storage. Aims The aim of this study was to examine responses of Norway spruce (Picea abies (L.) Karst.) to a range of SD treatments of different length and evaluate gene expression related to dormancy induction and development of freezing tolerance.

    Methods: The seedlings were SD treated for 11 h a day during 7, 14, 21, or 28 days. Molecular tests were performed, and the expression profiles of dormancy and freezing tolerance- related genes were analyzed as well as determination of shoot growth, bud set, bud size, reflushing, dry matter content, and timing of spring bud break.

    Results: The 7-day SD treatment was as effective as longer SD treatments in terminating apical shoot growth. However, short (7 days) SD treatment resulted in later activation of dormancy-related genes and of genes related to freezing tolerance compared to the longer treatments which had an impact on seedling phenology.

    Conclusion: Gene expression analysis indicated an effective stimulus of dormancy-related genes when the SD treatment is prolonged for at least 1-2 weeks after shoot elongation has terminated and that seedlings thereafter are exposed to ambient outdoor climate conditions.

  • 62.
    Wallin, Elisabeth
    et al.
    Dalarna University, School of Technology and Business Studies, Energy Technology.
    Gräns, Daniel
    Stattin, Eva
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Verhoef, Nathalie
    Mikusiński, Grzegorz
    Lindström, Anders
    Evaluating methods for storability assessment and determination of vitality status of container grown Norway spruce transplants after frozen storage2019In: Scandinavian Journal of Forest Research, ISSN 0282-7581, E-ISSN 1651-1891Article in journal (Refereed)
    Abstract [en]

    Autumn sown small seedlings for later transplanting into large containers have been introduced in Swedish forest tree nurseries. Containerized transplants of Norway spruce (Picea abies (L.) Karst.) from three Swedish nurseries were frozen stored during the autumn of 2014 to find out storability and post-storage vitality. Seedling storability was determined by measuring electrolyte leakage after freezing shoots to −25°C (SELdiff−25), by measurements of dry matter content (DMC) of seedling shoots and by the commercial molecular test ColdNSure™. Vitality of seedlings after storage was determined by measuring the leakage of electrolytes from shoots (SEL), and seedlings were also tested in regrowth tests. All three methods for storability assessment gave similar predictions, except in one case where DMC showed “not storable” for successfully stored seedlings. Our results indicated that young transplants can be successfully short term stored before reaching the target levels for safe long-term storage of conventional seedlings. Early storage of young transplants resulted in low post-storage survival and vitality expressed as root growth capacity and shoot electrolyte leakage (SEL). A prolonged duration in storage generally resulted in lower survival as well as lower root growth capacity and higher levels of SEL, especially for seedlings stored at earlier dates. 

  • 63. Wennström, Ulfstand
    et al.
    Johansson, Karin
    Lindström, Anders
    Stattin, Eva
    Dalarna University, School of Technology and Business Studies, Forest and Wood Technology.
    Produktion av frö och plantor: Skogsskötselserien del 22008In: Skogsskötselserien, Jönköping: Skogsstyrelsens förlag , 2008Chapter in book (Other academic)
12 51 - 63 of 63
CiteExportLink to result list
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  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
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  • Other locale
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  • text
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