Short-day photoperiods affect expression of genes related to dormancy and freezing tolerance in Norway spruce seedlingsShow others and affiliations
2017 (English)In: Annals of Forest Science, ISSN 1286-4560, E-ISSN 1297-966X, Vol. 74, no 3, article id 59Article in journal (Refereed) Published
Abstract [en]
Key message: Gene expression analysis showed that prolonged short day (SD) treatment deepened dormancy and stimulated development of freezing tolerance of Picea abies seedlings. Prolonged SD treatment also caused later appearance of visible buds in autumn, reduced risks for reflushing, and promoted earlier spring bud break.
Context: Short day (SD) treatment of seedlings is a common practice in boreal forest tree nurseries to regulate shoot growth and prepare the seedlings for autumn planting or frozen storage. Aims The aim of this study was to examine responses of Norway spruce (Picea abies (L.) Karst.) to a range of SD treatments of different length and evaluate gene expression related to dormancy induction and development of freezing tolerance.
Methods: The seedlings were SD treated for 11 h a day during 7, 14, 21, or 28 days. Molecular tests were performed, and the expression profiles of dormancy and freezing tolerance- related genes were analyzed as well as determination of shoot growth, bud set, bud size, reflushing, dry matter content, and timing of spring bud break.
Results: The 7-day SD treatment was as effective as longer SD treatments in terminating apical shoot growth. However, short (7 days) SD treatment resulted in later activation of dormancy-related genes and of genes related to freezing tolerance compared to the longer treatments which had an impact on seedling phenology.
Conclusion: Gene expression analysis indicated an effective stimulus of dormancy-related genes when the SD treatment is prolonged for at least 1-2 weeks after shoot elongation has terminated and that seedlings thereafter are exposed to ambient outdoor climate conditions.
Place, publisher, year, edition, pages
Springer France , 2017. Vol. 74, no 3, article id 59
Keywords [en]
Picea abies, Molecular tests, Photoperiod, Shoot growth termination, Bud formation. Storability
National Category
Biological Sciences
Research subject
Research Profiles 2009-2020, Energy and Built Environments
Identifiers
URN: urn:nbn:se:du-25920DOI: 10.1007/s13595-017-0655-9ISI: 000407478600012Scopus ID: 2-s2.0-85026841036OAI: oai:DiVA.org:du-25920DiVA, id: diva2:1137303
2017-08-312017-08-312025-10-09Bibliographically approved
In thesis