Understanding travel behavior and identifying the mode of transportation are essential for adequate urban devising and transportation planning. Global positioning systems (GPS) tracking data is mainly used to find human mobility patterns in cities. Some travel information, such as most visited location, temporal changes, and the trip speed, can be easily extracted from GPS raw tracking data. GPS trajectories can be used as a method to indicate the mobility modes of commuters. Most previous studies have applied traditional machine learning algorithms and manually computed data features, making the model error-prone. Thus, there is a demand for developing a new model to resolve these methods' weaknesses. The primary purpose of this study is to propose a semi-supervised model to identify transportation mode by using a contemporary machine learning algorithm and GPS tracking data. The model can accept GPS trajectory with adjustable length and extracts their latent information with LSTM Autoencoder. This study adopts a deep neural network architecture with three hidden layers to map the latent information to detect transportation mode. Moreover, different case studies are performed to evaluate the proposed model's efficiency. The model results in an accuracy of 93.6%, which significantly outperforms similar studies.